Journal: Molecular Cytogenetics

Article Title: Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

doi: 10.1186/s13039-020-00511-4

Figure Lengend Snippet: a Results of mFISH using all 21 murine whole chromosome paints as probes applied on murine MM cell line AB1 are shown here. b Typical pseudocolorbanding-results for murine multicolor banding (mcb) as applied on cell line AB1 for chromosomes 1 (mcb1), 2 (mcb2) and 6 (mcb6). Derivative chromosomes are shown as 1/2 a = der(1)t(1;2)(H5;F1), 1/2 b , = der(1)t(1;2)(C1;F1), 2/6 = der(2)(2A1→2H4::6F3~G1→6E1::6F3~G1→6qter) and 6/2 = der(6)t(2;6)(H1;E1); normal chromosomes are labeled by # and chromosome number

Article Snippet: The murine cell lines AB1 and AC29 were obtained from Cell Bank Australia (Westmead, Australia, order #s CBA-0144 and CBA-0152) and AB22 European Collection of Authenticated Cell Cultures (Salisbury, UK—order# ECACC 10092307).

Techniques: Labeling

Journal: Molecular Cytogenetics

Article Title: Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

doi: 10.1186/s13039-020-00511-4

Figure Lengend Snippet: aCGH results for cell line AB1. In a copy number variations detected are summarized with respect to a tetraploid karyotype. Gains are depicted as green bars (one more copy = light green; two more copies = dark green), loss of one copy is depicted as a red bar and loss of two copies is depicted as a dark-red bar. Breaks are registered here as arrows. In b results of in silico translation for AB1 to human genome are shown the same way as in a

Article Snippet: The murine cell lines AB1 and AC29 were obtained from Cell Bank Australia (Westmead, Australia, order #s CBA-0144 and CBA-0152) and AB22 European Collection of Authenticated Cell Cultures (Salisbury, UK—order# ECACC 10092307).

Techniques: In Silico

Journal: Molecular Cytogenetics

Article Title: Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

doi: 10.1186/s13039-020-00511-4

Figure Lengend Snippet: Comparison of ‘translated’ imbalances of murine MM-cellines AB1, AB22 and AC29 with human epitheloid, biphasic and sarcomatoid MM, according to Krismann et al. [ 22 ]

Article Snippet: The murine cell lines AB1 and AC29 were obtained from Cell Bank Australia (Westmead, Australia, order #s CBA-0144 and CBA-0152) and AB22 European Collection of Authenticated Cell Cultures (Salisbury, UK—order# ECACC 10092307).

Techniques: Comparison

Journal: Molecular Cytogenetics

Article Title: Cytogenomic characterization of three murine malignant mesothelioma tumor cell lines

doi: 10.1186/s13039-020-00511-4

Figure Lengend Snippet: The four tumor suppressor genes most often involved in human MM acc. to [ 7 – 16 ], being deleted and/or mutated there are compared for copy number variant observed in the three studied cell lines

Article Snippet: The murine cell lines AB1 and AC29 were obtained from Cell Bank Australia (Westmead, Australia, order #s CBA-0144 and CBA-0152) and AB22 European Collection of Authenticated Cell Cultures (Salisbury, UK—order# ECACC 10092307).

Techniques: Variant Assay

Journal: Journal of Translational Medicine

Article Title: FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma

doi: 10.1186/s12967-021-03089-6

Figure Lengend Snippet: FAM83A overexpression in HN4 and HN6 cells promotes cell growth and metastasis. A , B FAM83A expressions based on real-time PCR and western blotting in HN4 and HN6 cell lines after treated with LV-FAM83A. C Morphological changes of HN4 and HN6 cells after LV-FAM83A transfection (100 ×). D CCK8 assays of the proliferation in HN4 and HN6 cells after LV-FAM83A transfection. E , F A wound-healing assay in HN4 and HN6 cells after LV-FAM83A transfection (100 ×). G , H Transwell assays of migration and invasion in HN4 and HN6 cells after LV-FAM83A transfection (100 ×). Data represent the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The human normal oral keratinocytes (HOK) and the human HNSCC cell lines including CAL27, FADU, HN4, HN6 SCC-9, and SCC-25 cell, were purchased from China Center for Type Culture Collection (Shanghai, China), CAL27 and HOK were cultured in DMEM medium (Gibco).

Techniques: Over Expression, Real-time Polymerase Chain Reaction, Western Blot, Transfection, Wound Healing Assay, Migration

Journal: Journal of Translational Medicine

Article Title: FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma

doi: 10.1186/s12967-021-03089-6

Figure Lengend Snippet: FAM83A promotes EMT and activates Wnt/β-catenin signaling pathwayin HNSCC cells. A Protein levels of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and Wnt-responsive genes including β-catenin, phosphor-β-catenin, c-myc and Cyclin D1 were determined by western blotting in CAL27 and FADU cells after shFAM83A transfection. B , C Gene expressions of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail, c-myc and Cyclin D1 were measured by RT-PCR in CAL27 and FAUD cells after shFAM83A transfection. D Protein levels of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and Wnt-responsive gene including β-catenin, phosphor-β-catenin, c-myc and Cyclin D1 were determined by western blotting in HN4 and HN6 cells after LV-FAM83A transfection. E , F Gene expressions of FAM83A, E-cadherin, N-cadherin, Vimentin, Snail and c-myc and Cyclin D1 were measured by RT-PCR in HN4 and HN6 cells after LV-FAM83A transfection. G Immunofluorescence analysis of the EMT marker E-cadherin and Vimentin in CAL27 cells after shFAM83A transfection (200 ×). H Immunofluorescence analysis of the EMT marker E-cadherin and Vimentin in HN6 cells after LV-FAM83A transfection (200 ×). Data represent the mean ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The human normal oral keratinocytes (HOK) and the human HNSCC cell lines including CAL27, FADU, HN4, HN6 SCC-9, and SCC-25 cell, were purchased from China Center for Type Culture Collection (Shanghai, China), CAL27 and HOK were cultured in DMEM medium (Gibco).

Techniques: Western Blot, Transfection, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Marker